What do you most frequently have to guard against in terms of general system configuration or changes causing what look like defects in your product’s code?
One frequent source of issues I can think of: the popup blocker whose activity is recorded BUT well-hidden in the status bar of the browser.
Also: changes to Windows security policies either from your IT department or through Windows system updates.
I would add the following points to this question :
Black-listing of IP addresses on some network providers
switching from Windows to Mac
browsers - some issues are browser-specific
device issue - especially in the case of Android devices for mobile applications, as every Android device manufacturer has modified the Android so which also causes issues some time